Rupanyup have worn the traditional tripanel royal blue and red guernsey design for as long as anyone cares to remember. When the club was admitted to the Horsham District League this guernsey caused a considerable clash with fierce rivals Kalkee who wear guernseys that are predominantly red with a royal blue sash. After many years through the 80s and 90s and trialling measures such as wearing yellow socks to better distinguish each side it was decided in 2001 to wear a navy blue clash strip for all games against Kalkee whilst retaining the traditional Rupanyup strip for all other games. The navy blue colours of the clash strip honour the legacy of the neighbouring Marnoo Football Club that went into recess in the year 2000 and were worn in Rupanyup's most recent Senior premiership against Kalkee in 2001 and again in the Reserves premiership in 2009.